Sequences from confirmation amplicon


Confirmation sequences for line 402D05 and hit At5g20730 are shown.
Line ID 402D05
Locus At5g20730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7022004
Primers used for PCR HM13 - 8474, show primer details
   
Sequence Name 09-K028918-022-402-D05C-8409
GenBank Accession LN490043 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K028918-022-402-D05C-8409
GATTTTCAGGGATGACCAAGGACAATCGAAGATTAAGACTCAAACGTGAGAGAAAGAGCA
ACAGAGTTGTCGGAAAGGGATGATCAGCGACCAGGACAACTACCAAAAGGTCTGCTAAAG
CACAGGAACAGCTTTTTCTTCTTCCTCTCCTTTTTTTTTCCTTCTCTTTTCTTTTTCCGA
TATTTTTATTTTTTTATACCTATTTAGTTATGCTCATGAAGCCGACATATTCACTAGCAA
ACTGGCCACGATTTAATTTCTTAATGTTATTATTGTCCTCTAAAATTTTAACTTTGACAC
ACAAAAAAACAACTTTATTAGTGTTTTCTTTTTCCTTATCTCTGATAATTTGATAAGTTT
TTTTCATTAAGGTTATTATTACTGTATCATTTATTGTAATACTTGATTAGGGATAACATA
GATTTATAGGGGATGTATTGAAAAAAACAAACTAAGCTGACTAGCTAGTTATTTGATCAA
AGTGCGTACATATATGTTTGGCAATTTCTTTTGTAGTTAACTATATTTAATTCAGATAGA
TTTTAGGATTCATAATTCATCAAAAAATTTTATGATTTTCATTATTTTGAACAAAAGCAT
TTTACTAGAAATTTTGTGGAGTTTT
   
Sequence Name 10-K028918-022-402-D05C-HM13
GenBank Accession LN490517 [GenBank]
Sequencing primer gene specific primer, HM13: 5'-AGAACCAACCATGTAAAACTCCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K028918-022-402-D05C-HM13
AATAATGAAAATCATAAAATTTTTTGATGAATTATGAATCCTAAAATCTATCTGAATTAA
ATATAGTTAACTACAAAAGAAATTGCCAAACATATATGTACGCACTTTGATCAAATAACT
AGCTAGTCAGCTTAGTTTGTTTTTTTCAATACATCCCCTATAAATCTATGTTATCCCTAA
TCAAGTATTACAATAAATGATACAGTAATAATAACCTTAATGAAAAAAACTTATCAAATT
ATCAGAGATAAGGAAAAAGAAAACACTAATAAAGTTGTTTTTTTGTGTGTCAAAGTTAAA
ATTTTAGAGGACAATAATAACATTAAGAAATTAAATCGTGGCCAGTTTGCTAGTGAATAT
GTCGGCTTCATGAGCATAACTAAATAGGTATAAAAAAATAAAAATATCGGAAAAAGAAAA
GAGAAGGAAAAAAAAAGGAGAGGAAGAAGAAAAAGCTGTTCCTGTGCTTTAGCAGACCTT
TTGGTAGTTGTCCTGGTCGCTGATCATCCCTTTCCGACAACTCTGTTGCTCTTTCTCTCA
CGTTTGAGTCTTAATCTTCGATTGTCCTTGGTCATCCTGAAATCTATTCATTTTAAACCA
TCATACATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAT
TATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCG
AAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT

Last Updated on 06.12.2018 16:11