Sequences from confirmation amplicon


Confirmation sequences for line 406D08 and hit At2g06510 are shown.
Line ID 406D08
Locus At2g06510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:2586416
Primers used for PCR HP44 - 8474, show primer details
   
Sequence Name 69-K033553-022-406-D08C-8409
GenBank Accession LN512131 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K033553-022-406-D08C-8409
GCCATTTACAATTGAATATTTGGCAATTCGGGTTACCTCCCCTCCATCATAGTCAAGCAG
ATCAAACGAGAAAACCTTTCCATCTCCTTTTGCATTGTTATATCGTCTGATATCTCCTTT
CGCAGTTACTCTAGCCTTAATAGCCCATCGACCTTGGTATGGATTGAGAGCAGCAATAGG
GATGACTCGAGCTGGAGCCTCGTTCTTCATGATGGGTCCATGATTTCTGTAACTTGCAGG
CGGTTGATATGAAGGTTGAACAGATGGCCTGAAGCTTGGAGTATTGTTGGCTTGATTTTG
AATGTTTACACCCCTGGGAGGTGCTCTATTAACAGAATG
   
Sequence Name 69-K033553-022-406-D08C-HP44
GenBank Accession LN512132 [GenBank]
Sequencing primer gene specific primer, HP44: 5'-GCATTCTGTTAATAGAGCACCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K033553-022-406-D08C-HP44
ATCAGCCACATACTCCAAGCTTCAGGCCATCTGTTCAACCTTCATATCAACCGCCTGCAA
GTTACAGAAATCATGGACCCATCATGAAGAACGAGGCTCCAGCTCGAGTCATCCCTATTG
CTGCTCTCAATCCATACCAAGGTCGATGGGCTATTAAGGCTAGAGTAACTGCGAAAGGAG
ATATCAGACGATATAACAATGCAAAAGGAGATGGAAAGGTTTTCTCGTTTGATCTGCTTG
ACTATGATGGAGGGGAGGTAACCCGAATTGCCAATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATG
AAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATA
AACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCCAAATGG

Last Updated on 06.12.2018 16:11