Sequences from confirmation amplicon


Confirmation sequences for line 440H11 and hit At4g14540 are shown.
Line ID 440H11
Locus At4g14540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8344632
Primers used for PCR PG16 - 8474, show primer details
   
Sequence Name 53-K101746-0022-440-H11C-8409
GenBank Accession LN508102 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K101746-0022-440-H11C-8409
ATTTACATTGAATATATCCTGATTATTATCCTGATTTCTCGACCTTTTGTCTCCTCGATC
TAAAGATGGCGGATTCGGACAACGATTCAGGAGGACACAAAGACGGTGGAAATGCTTCGA
CACGTGAGCAAGATAGGTTTCTACCGATCGCTAACGTTAGCAGGATCATGAAGAAAGCAC
TTCCTGCGAACGCAAAAATCTCTAAGGATGCTAAAGAAACGGTTCAAGAGTGTGTATCGG
AATTCATAAGTTTCATCACCGGTGAGGCTTCTGACAAGTGTCAGAGAGAGAAGAGGAAGA
CAATCAACGGTGACGATCTTCTTTGGGCGATGACTACGCTAGGGTTTGAGGACTACGTGG
AGCCTCTCAAGGTTTATCTGCAAAAGTATAGGGAGGTGGAAGGAGAGAAGACTACTACGG
C
   
Sequence Name 53-K101752-0022-440-H11C-PG16
GenBank Accession LN508103 [GenBank]
Sequencing primer gene specific primer, PG16: 5'-TGCCGTAGTAGTCTTCTCTCCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K101752-0022-440-H11C-PG16
ATACCTTGAGAGCTCCCGTAGTCCTCAAACCCTAGCGTAGTCATCGCCCAAAGAAGATCG
TCACCGTTGATTGTCTTCCTCTTCTCTCTCTGACACTTGTCAGAAGCCTCACCGGTGATG
AAACTTATGAATTCCGATACACACTCTTGAACCGTTTCTTTAGCATCCTTAGAGATTTTT
GCGTTCGCAGGAAGTGCTTTCTTCATGATCCTGCTAACGTTAGCGATCGGTAGAAACCTA
TCTTGCTCACGTGTCGAAGCATTTCCACCGTCTTTGTGTCCTCCTGAATCGTTGTCCGAA
TCCGCCATCTTTAGATCGAGGAGACAAAAGGTCGAGAAATCAGGATAATAATCAGGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCG

Last Updated on 06.12.2018 16:11