Sequences from confirmation amplicon


Confirmation sequences for line 445E03 and hit At5g52600 are shown.
Line ID 445E03
Locus At5g52600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21344101
Primers used for PCR HV15 - 8474, show primer details
   
Sequence Name 17-K031868-022-445-E03C-8409
GenBank Accession LN493740 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K031868-022-445-E03C-8409
CCATTTACAATTGGTAATTACATGTAGTTAAATGGTAGCATTTGAGTTTGAAGCCATTTC
CGACAGTAACACTACTGTAAATTCGTGCAAACTAGAGCTGTAAAAGCTCTATTCCCTTGT
TTTTATCCTCAACATTGTCACAAATTATTGGCTTTTAAGGCAAACATATAAGCACGGGAG
ATCAGAGGTTGAAGATTGCCATCAACCTCGATACATGCGTTAGTCCATTGCATTGCAGAT
ACAGTTTGTGCGTTTACAGTTGTTGCGTTTCAAATATTCTTTCTCGCTCAAAATGCTTTT
CCAATAGTCAAGACTAGTGTATAAGGGATAACAAATCAATATCTAAAAAATCTAAGAACG
AAAAGAGAGGTATATGTCTAATATACATATTTGAGTGATATATGCATCTACGTCCTAGCT
AGTTCTTAGTTTTCAGATTTTAATATTATGGAATTTGGTAACTTACATTTAAAAAGAAAA
CACGATTCAACTTATTTTCTTTTTTAATATGATATCATAAGATACACCACGATTAAAATC
TGAAAATTCCATATAGGTCAATAACTTTCGAAAAACATTATAGTACCAAAAGATAATTAT
TACAACAAGAAACCATCTTTTTATTTTGTGTAACTTT
   
Sequence Name 18-K031868-022-445-E03C-HV15
GenBank Accession LN494189 [GenBank]
Sequencing primer gene specific primer, HV15: 5'-TGAGCCAAACAAAAGTTACACAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K031868-022-445-E03C-HV15
TGTATATTATCTTTTGGTACTATAATGTTTTTCGAAAGTTATTGACCTATATGGAATTTT
CAGATTTTAATCGTGGTGTATCTTATGATATCATATTAAAAAAGAAAATAAGTTGAATCG
TGTTTTCTTTTTAAATGTAAGTTACCAAATTCCATAATATTAAAATCTGAAAACTAAGAA
CTAGCTAGGACGTAGATGCATATATCACTCAAATATGTATATTAGACATATACCTCTCTT
TTCGTTCTTAGATTTTTTAGATATTGATTTGTTATCCCTTATACACTAGTCTTGACTATT
GGAAAAGCATTTTGAGCGAGAAAGAATATTTGAAACGCAACAACTGTAAACGCACAAACT
GTATCTGCAATGCAATGGACTAACGCATGTATCGAGGTTGATGGCAATCTTCAACCTCTG
ATCTCCCGTGCTTATATGTTTGCCTTAAAAGCCAATAATTTGTGACAATGTTGAGGATAA
AAACAAGGGAATAGAGCTTTTACAGCTCTAGTTTGCACGAATTTACAGTAGTGTTACTGT
CGGAAATGGCTTCAAACTCAAATGCTACCATTTAACTACATGTAATTACCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAT
TATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCG
AAAGCAATAAACAAATTATTCTAATTCGGAAATCTNTATNTCGACGTGTC
Primers used for PCR FS08 - 3144, show primer details
   
Sequence Name 52-K033041-022-445-E03C-3144
GenBank Accession LN507722 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K033041-022-445-E03C-3144
TCCACATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTTCCCGAACGGATGTGAGGACGACGTTGTTGCTGTGGTGGG
CCTTTTTCTACATTTACATTTTTTCTTTTTCTCTTCAATAATTTTAGTAGATAGTCCCCA
CATGTAAGAGTTAAAGTTGGAAACCTCAAACTTTTTGTGTATATAGTTAATAAACAGCCT
CACTCACTTGCAAAACTGTGGAAGAAAAAATAAAAGATGGAATGCAAAAGAGAAGAAGGG
AAGTCTTACGTGAAGAGAGGGTTGTGGAAACCAGAAGAAGATATGATATTAAAAAGCTAT
GTTGAGACTCATGGTGAAGGAAACTGGGCAGACATTTCTCGTAGATCCGGTTCGATTTCC
AAATTTTTGTGTTTACTAGTAAAGGGGAGATGGGATTTTGGGACATTAAAGAATTTGTGT
TTCTGCATGATGTTTTTTTCAGGGTTGAAGAGAGGAGGAAAAAGCTGTAGGCTGAGATGG
AAGAACTATCTAAGACCAAATATCAAAAGAGGAAGCATGTCACCACAAGAACAAGACCTT
ATCATCCGCATGCATAAGCTTCTTGGAAACAGGTCTTTCCT
   
Sequence Name 20-K033041-022-445-E03C-FS08
GenBank Accession LN495112 [GenBank]
Sequencing primer gene specific primer, FS08: 5'-GATCAGTGGTGAAAGAGGAAAGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K033041-022-445-E03C-FS08
CTATGCATGCGGAGATAAGGTCTTGTTCTTGTGGTGACATGCTTCCTCTTTTGATATTTG
GTCTTAGATAGTTCTTCCATCTCAGCCTACAGCTTTTTCCTCCTCTCTTCAACCCTGAAA
AAAACATCATGCAGAAACACAAATTCTTTAATGTCCCAAAATCCCATCTCCCCTTTACTA
GTAAACACAAAAATTTGGAAATCGAACCGGATCTACGAGAAATGTCTGCCCAGTTTCCTT
CACCATGAGTCTCAACATAGCTTTTTAATATCATATCTTCTTCTGGTTTCCACAACCCTC
TCTTCACGTAAGACTTCCCTTCTTCTCTTTTGCATTCCATCTTTTATTTTTTCTTCCACA
GTTTTGCAAGTGAGTGAGGCTGTTTATTAACTATATACACAAAAAGTTTGAGGTTTCCAA
CTTTAACTCTTACATGTGGGGACTATCTACTAAAATTATTGAAGAGAAAAAGAAAAAATG
TAAATGTAGAAAAAGGCCCACCACAGCAACAACGTCGTCCTCACATCCGTTCGGGAAATC
TGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGT
CTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAAT
CCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGT

Last Updated on 06.12.2018 16:11