Sequences from confirmation amplicon


Confirmation sequences for line 469C11 and hit At1g20700 are shown.
Line ID 469C11
Locus At1g20700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7182865
Primers used for PCR NU30 - 8474, show primer details
   
Sequence Name 48-K103927-0022-469-C11C-NU30
GenBank Accession LN506489 [GenBank]
Sequencing primer gene specific primer, NU30: 5'-TAACCCTCAAATCAGCTTACGTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K103927-0022-469-C11C-NU30
TATTGAATTGTTCGATCTAAGCAAATCATAAACTATATTAACAAATATCTTTAGCAATGA
TAAATCGATATCTAAGCGGTAGGTAAACTGTCTTCGAATGTCTAAAACATTATGAAATTT
GATTCAAAGTTATGCATCGAACACTGGGAAAAAAAAAGCCTTGGATTTCGTTAGTTGTAT
ACCAAACGCAAACACAATTTTCACATTTTTACACTAAAGTCTCAATGGGTCAAGTGTATT
AATGGAAACAACGTTTGCATCGCAAAAAACGCTGCGTTTAAACACTTTCGTCTTACACAC
TTCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAAT
   
Sequence Name 64-K103928-0022-469-C11C-8409
GenBank Accession LN511190 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K103928-0022-469-C11C-8409
CATTTACAATTGAAGAAGTGTGTAAGACGAAAGTGTTTAAACGCAGCGTTTTTTGCGATG
CAAACGTTGTTTCCATTAATACACTTGACCCATTGAGACTTTAGTGTAAAAATGTGAAAA
TTGTGTTTGCGTTTGGTATACAACTAACGAAATCCAAGGCTTTTTTTTTCCCAGTGTTCG
ATGCATAACTTTGAATCAAATTTCATAATGTTTTAGACATTCGAAGACAGTTTACCTACC
GCTTACATATCAATTTATCATTGCTAACGATATTTGTCCATATATTTTATGATTCTGCTT
GGATCACACAATTCACCATTTTATCAATGTC

Last Updated on 06.12.2018 16:11