Sequences from confirmation amplicon


Confirmation sequences for line 481C08 and hit At2g02950 are shown.
Line ID 481C08
Locus At2g02950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:856436
Primers used for PCR CF08 - 8474, show primer details
   
Sequence Name 41-K025447-022-481-C08C-8409
GenBank Accession LN503936 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K025447-022-481-C08C-8409
TCCCAAAAACATCTTTTGATTTCATGAAGAATAACAACAGTCACAGCAGTCTCTATGTTT
CTTCTTCTTCTTACTTGAGCAGCAAGGAAGATGCTCTTGTCACGACCAAGAAGCTCATGG
AACCAAGCAAAACACTAAACATGAGCATTAATCCAAAACAAGAGGAGTTTGGTGATGAGA
AGAAAATGGTGAAGAAAGCTCCTGAAGATCCAGAGATTGGTGTGTTTGGAGCTGAGAAGT
ACTTCAATGGAGACATGGATTCAGACCAAGGTTCTAGTGTTCTGTCTCTGACAAACCCAG
AAGTTGAGAGAACCGTCGTCGACTCGAAGCAGAGCGCGAAGAAATCTACTGGTACTCCGA
GTGTCCGGTCTGAATCAAGCTGGAATAGTCAGAGCGTGTTGCTTCAGAACAAACTGGTGA
ATAGCTGCAACA
   
Sequence Name 42-K025447-022-481-C08C-CF08
GenBank Accession LN504323 [GenBank]
Sequencing primer gene specific primer, CF08: 5'-ACTGGAGATCCAAAAACTTCAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K025447-022-481-C08C-CF08
TGATAACTCCTCTTGCTTCTGAATCTTGATCAGTTCTGTGTTCATATCCGCAGAAGATCT
CATTGTGATAACAGAGATATTCGGATCAGCGCTTCTCTTGACCGAGGTTTTCTCCTCGAC
ATCTACAGAATCCCCATCAGAGCATGCGCATTTACACCCCAAATTTGCGAGAAAACTCTT
CTTATTATTGGTCACCTTTTGAATCTGACCATTACTGTTCTTCTTTTCCTTGAAGGAACT
GTTGCAGCTATTCACCAGTTTGTTCTGAAGCAACACGCTCTGACTATTCCAGCTTGATTC
AGACCGGACACTCGGAGTACCAGTAGATTTCTTCGCGCTCTGCTTCGAGTCGACGACGGT
TCTCTCAACTTCTGGGTTTGTCAGAGACAGAACACTAGAACCTTGGTCTGAATCCATGTC
TCCATTGAAGTACTTCTCAGCTCCAAACACACCAATCTCTGGATCTTCAGGAG
Primers used for PCR CT33 - 8474, show primer details
   
Sequence Name 44-K118809-0022-481-C08C-CT33
GenBank Accession LN505347 [GenBank]
Sequencing primer gene specific primer, CT33: 5'-ATTATTCTCCATTGATTGGCAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K118809-0022-481-C08C-CT33
AATAATCAGTTCCACACACAACAACTGGCCAATGACTCAAACTGGAACCCACTCACATTG
ATCAGATCATAACACTTTTAGCTTCAGAAAAAAAAAAAAAAACGGCTTTCAAACATATCG
CCTGGTTACCCTTTTTAACCCCAATTTCCCCTTTTCTCCCCCCAGGCATTAAGGAGAGAA
AAAATTTTAAGGCTCCCAAAAAAAAAGGGGGGACCCTAACCCCACCATTTGAAAAGGGCT
TCTGGCCCGGGAAATCTCCGGGGATCACCATGGATTATGAGGGTCAATTGGGAAAAAAAA
AAAGAGTATTTCCCATTTTTTTTTCATTTCAAAAAGGTAAATTCCCCCACACTTAAAAAA
AAAAAAA
   
Sequence Name 12-K118808-0022-481-C08C-8409
GenBank Accession LN491807 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K118808-0022-481-C08C-8409
TTACAATTGATGGTGTTAGTGTCACCATTTTCTCTTTGGTAGCTTTATATTTTTTTCTCT
CTTTATTGCTTGTGTGTAGATATGTGGAATTAGGGTATAAATAGAGTAACAAGACGATAT
GTTTGATAGCTGTTTTTTTTTTTTTTCCGAAACCAAAAGGGGTTATAACCGGACCAAGGG
GAGGGGGTCCCATTTGGAGCCATGGCCCATTTGTGGGGGGGGGAACCGGATTTATTTAAA
TTTTGGGAAAAGGAACCTTGCCCAACCAGGGGAAAAAAAAA

Last Updated on 06.12.2018 16:11