Sequences from confirmation amplicon


Confirmation sequences for line 485F07 and hit At1g20840 are shown.
Line ID 485F07
Locus At1g20840 [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7246530
Primers used for PCR JT03 - 8474, show primer details
   
Sequence Name 29-K026134-022-485-F07C-8409
GenBank Accession LN499137 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined)
>29-K026134-022-485-F07C-8409
GTAGATTTTCCCGGACATGAAGCCACTCCGAGTGTTTCCGCCTGCTTCTGGCATCTTCTC
GTGGAGACTGCCAAAAAGATTGACGAGCGGATCTTTAAGGATCATGCTTTGGTTTGCTAA
GCTTCCGTGGCGAGAGCGTAGCCCAAGTGAGCTATTTTGTTCTGGGACAGGTCTAGCAAG
GTACGATTGATTCTCGTGGGTTCCATAAAGCCGCATTTGTCCATCCTCATCAACGGTTTC
AAGTGTATCATCACCTTCATGATCCTCCAAAGTTACTAAGAGATCTTCCATTGTTTTTTC
TCCTCCTATATCTAGTCCTTCAACTAGTAAAGCCATCTCACTTGCATT
   
Sequence Name 30-K026134-022-485-F07C-JT03
GenBank Accession LN499574 [GenBank]
Sequencing primer gene specific primer, JT03: 5'-AAATGCAGATGAGATGGCTTTACT-3'
Sequence (A. th genome BLAST matches underlined)
>30-K026134-022-485-F07C-JT03
TGAAGGACTAGATATAGGAGGAGAAAAAACAATGGAAGATCTCTTAGTAACTTTGGAGGA
TCATGAAGGTGATGATACACTTGAAACCGTTGATGAGGATGGACAAATGCGGCTTTATGG
AACCCACGAGAATCAATCGTACCTTGCTAGACCTGTCCCAGAACAAAATAGCTCACTTGG
GCTACGCTCTCGCCACGGAAGCTTAGCAAACCAAAGCATGATCCTTAAAGATCCGCTCGT
CAATCTTTTTGGCAGTCTCCACGAGAAGATGCCAGAAGCAGGCGGAAACACTCGGAGTGG
CTTCATGTCCGGGAAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
ATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGA
AAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 23.10.2014 13:25