Sequences from confirmation amplicon


Confirmation sequences for line 511A03 and hit At1g01080 are shown.
Line ID 511A03
Locus At1g01080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:46901
Primers used for PCR 42hw - 8474, show primer details
   
Sequence Name 54-K112667-0022-511-A03C-42hw
GenBank Accession LN508528 [GenBank]
Sequencing primer gene specific primer, 42hw: 5'-AGATTACAGTTCCAAAAGGCTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K112667-0022-511-A03C-42hw
CTTCAGGGATATACCACGTAGAGCTCGCAGGTCTCGGTTTCTTTACTGGTTCTGCTTTGG
AGACGACACTATCTTTATTGGGTTCTGCTTCTCCTTTAACTACTTCTTCTTCTTTTTCTT
CATCGAGAACTGATAATTCTGAAGAAGAGACCTTGATTTGAAGTGGTTGAGCTACAAAGG
GACGAGAAAGAGAAGAGTGATGAAAAGAATTGGAAGAAGAGAATTGAAGTTTCAGAGATT
CTAAGTAAGAGTAAGAAGAAGAAGAGATTAGGGTTTTGTATTTGGGAATTGCATTGTGAG
ACGATCGAGAAGAAGAAGATAAGGGAATTGCGAAGCAGGAGGCCGCCATTATCGAAGGAC
CAGAAAGTAAATTATTTGAGAAGAATGATTAAAAAAAAAAGAAATGAAATAGAATTTAAT
AGAACAAATCGGGTCGGGTCATTTAACCCGTTACAATCCTGATCATGGATCAGGATATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTT
CCGCA
   
Sequence Name 22-K112666-0022-511-A03C-8409
GenBank Accession LN496184 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K112666-0022-511-A03C-8409
CATTTCATTGAATATATCCTGATCCATGATCAGGATTGTAACGGGTTAAATGACCCGACC
CGATTTGTTCTATTAATTTCTATTTCATTTCTTTTTTTTTAATCATTCTTCTCAAATAAT
TTACTTTCTGGTCCTTCAATAATGGCGGCCTCCTGCTTCGCAATTCCCTTATCTTCTTCT
TCTCGATCGTCTCACAATGCAATTCCCAAATACAAAACCCTAATCTCTTCTTCTTCTTAC
TCTTACTTAAAATCTCTGAAACTTCAATTCTCTTCTTCCAATTCTTTTCATCACTCTTCT
CTTTCTCGTCCCTTTGTAGCTCAACCACTTCAAATCAAGGTCTCTTCTTCAAAATTATCA
GTTCTCGATGAAAAAAAAGAAAAAAAAGTATTTAAAGGAGAAGCAAAACCCAATAAAGAT
AGTGTCGTCTCCAAAGCAAAACCAGTAAAGAAACCGAGACCTTGCGAGCTCTACTTGTGT
AATATCCCTAGAAGCTACAACTTTGCTCAGCTTCTTGACATGTTTCACCCTTTTGGACGT
GTAAT

Last Updated on 06.12.2018 16:11