Sequences from confirmation amplicon


Confirmation sequences for line 511C08 and hit At1g59580 are shown.
Line ID 511C08
Locus At1g59580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21884784
Primers used for PCR GE53 - 8474, show primer details
   
Sequence Name 52-K101502-0022-511-C08C-8409
GenBank Accession LN507762 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K101502-0022-511-C08C-8409
GCCTTTACAATTGAATGTCGAAGATGACGTAGAAGCTTGAGCTCCCTAAGAGTCCTCAAC
GCATCAATCCTATTCTCAAACACATTGTGGATCTTCTTGATCGCCACTCTCTCATTACTC
TCTCTGTTAACCGAAGAGCAAACCACACCGTACGCGCCTCGGCCTATCGGTTTGATAGGC
ACGTATTTGGTATCGATCTCGAAAAGTGTTTGCCACATTGAGAAGTAATGCTTCCCTTGA
TTCCTAATTCCATTAGGTGGATCAACAGGAGTCGCCATTCTTCTACCTCCTTATAAAAAC
ACACAACAAAAGGAATAAAAATCAAAACTTTATCGAAATTGGAACAATCCACAAATTTGA
AAATATACACAACACAGAATTTTGAATTGCTTCCAACAAGTGACAATGAAAG
   
Sequence Name 52-K101506-0022-511-C08C-GE53
GenBank Accession LN507763 [GenBank]
Sequencing primer gene specific primer, GE53: 5'-TCTTTCATTGTCACTTGTTGGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K101506-0022-511-C08C-GE53
TTTTCAATTTGTGGATTGTTCCATTTCGATAAGTTTTGATTTTTATTCCTTTTGTTGTGT
GTTTTTATAAGGAGGTAGAAGAATGGCGACTCCTGTTGATCCACCTAATGGAATTAGGAA
TCAAGGGAAGCATTACTTCTCAATGTGGCAAACACTTTTCGAGATCGATACCAAATACGT
GCCTATCAAACCGATAGGCCGAGGCGCGTACGGTGTGGTTTGCTCTTCGGTTAACAGAGA
GAGTAATGAGAGAGTGGCGATCAAGAAGATCCACAATGTGTTTGAGAATAGGATTGATGC
GTTGAGGACTCTTAGGGAGCTCAAGCTTCTACGTCATCTTCGACATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11