Sequences from confirmation amplicon


Confirmation sequences for line 533H10 and hit At2g23760 are shown.
Line ID 533H10
Locus At2g23760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10108205
Primers used for PCR NF55 - 8474, show primer details
   
Sequence Name 10-K102498-0022-533-H10C-8409
GenBank Accession LN490600 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K102498-0022-533-H10C-8409
TTACAATTGAATATATACTCATTGCGGACGTGAATGTCATCGTCGTTGGAGTTTGTGCGG
TTATGACAGTGAAGTTGTTTTCATTGTTGTTGGGTTTCGTGTCGTTGTTGTTTGTTTGTT
GCTGTCTTCTTTGTTGTTGTTGATTTTCATTTTCTTCTTCTGCTTCTTCTCTTTCTTTTG
CTTCTTGTTGATACATCTCTTCCACCATTGGTTTCCATAGGCGAACCCTAGCATTTATGA
ACCAATTTGACACCTGAAAAAACGAGACATATGAACTAATATTAATCACAATTTACTCTT
AACAAGTTTATAATATTAAAAGAAACTTAACTTTTAGAAGGAAAAAAAAACTTTTCAATG
AAATGTGAAATACTCCTTTTTACATTAAATATGGTTTTGATTGTTTTTGTATATACTTCA
TTTGTTTTATATTAAGTAAAGTTAAAATAATAAGTCATTTACTGTGAAGAAAATTAGTTT
AAACAAATGAAACAAAAAAAATAGGATAGCTAAAACCTTAAGTTAAAATGATAGGAAAGG
AGTAAAAATTATGATACATGTGTTTCCCTTTG
   
Sequence Name 10-K102499-0022-533-H10C-NF55
GenBank Accession LN490601 [GenBank]
Sequencing primer gene specific primer, NF55: 5'-TCTCAAAGGAAACACATGTATCATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K102499-0022-533-H10C-NF55
TTTTACTAAGGTTTAGCTATCCTATTTTTTTTGTTTCATTTGTTTAAACTAATTTTCTTC
ACAGTAAATGACTTATTATTTTAACTTTACTTAATATAAAACAAATGAAGTATATACAAA
AACAATCAAAACCATATTTAATGTAAAAAGGAGTATTTCACATTTCATTGAAAAGTTTTT
TTTTCCTTCTAAAAGTTAAGTTTCTTTTAATATTATAAACTTGTTAAGAGTAAATTGTGA
TTAATATTAGTTCATATGTCTCGTTTTTTCAGGTGTCAAATTGGTTCATAAATGCTAGGG
TTCGCCTATGGAAACCAATGGTGGAAGAGATGTATCAACAAGAAGCAAAAGAAAGAGAAG
AAGCAGAAGAAGAAAATGAAAATCAACAACAACAAAGAAGACAGCAACAAACAAACAACA
ACGACACGAAACCCAACAACAATGAAAACAACTTCACTGTCATAACCGCACAAACTCCAA
CGACGATGACATTCACGTCCGCAATGAGTATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAG
TACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACA
AATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCCTCCCAAAA

Last Updated on 06.12.2018 16:11