Sequences from confirmation amplicon


Confirmation sequences for line 537H11 and hit At1g01780 are shown.
Line ID 537H11
Locus At1g01780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:283828
Primers used for PCR HM34 - 8474, show primer details
   
Sequence Name 47-K032522-022-537-H11C-8409
GenBank Accession LN506105 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K032522-022-537-H11C-8409
ATTTACATTGATCCATCATACTCCTTCCATTTGTATCTGTTACCAAATAACCCGATTAGA
ACCAAGCCAAAATTGATTCCACATTTGCTTGGACCAACCAACATCACGTTCTCAAATCGA
ACCAAAAGTATACTAAACCAAACCAAAATCAAAATTTTATTTCCATTTGATCTAGTAATG
ATTTTATCCGAACAGAAAAATTCTACGGAAATGAAAAAACAAAAAACAAATTCTAGAGCA
AATAAATTTCTCTACGAGACTAGTTCGTTGGTATTTTAGGCCATGAGAACATTTGCAGAT
GCGTATACCACTAACCTTTTCAAGAGGGTAAACAGTTTTTTCGCAAGCGGCACACTTGTC
TTGTGTTCCACAGAAGATGGAAGATATCTTGCTGGGAGTCCTANNTCTGTAA
   
Sequence Name 47-K032522-022-537-H11C-HM34
GenBank Accession LN506106 [GenBank]
Sequencing primer gene specific primer, HM34: 5'-ATTACAGACTAGGACTCCCAGCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K032522-022-537-H11C-HM34
GACAGTGTGCCGCTTGCGAAAAAACTGTTTACCCTCTTGAAAAGGTTAGTGGTATACGCA
TCTGCAAATGTTCTCATGGCCTAAAATACCAACGAACTAGTCTCGTAGAGAAATTTATTT
GCTCTAGAATTTGTTTTTTGTTTTTTCATTTCCGTAGAATTTTTCTGTTCGGATAAAATC
ATTACTAGATCAAATGGAAATAAAATTTTGATTTTGGTTTGGTTTAGTATACTTTTGGTT
CGATTTGAGAACGTGATGTTGGTTGGTCCAAGCAAATGTGGAATCAATTTTGGCTTGGTT
CTAATCGGGTTATTTGGTAACAGATACAAATGGAAGGAGTATGATGGATCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAT
TATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCG
AAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
Primers used for PCR FQ41 - 3144, show primer details
   
Sequence Name 09-K033040-022-537-H11C-FQ41
GenBank Accession LN490071 [GenBank]
Sequencing primer gene specific primer, FQ41: 5'-GCAACAACATTCATTCAATCATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K033040-022-537-H11C-FQ41
CAGACTCTGAACGCCATTCTCTTCCTTGGCCTCCACGGCGACGTCTTCGGTAGGTTCCGG
TGGAAGAGTGTTGCCTGAGGCTGTGCGACGGTGATTGGCGGCTTGGAGGACGTGAGCGTA
GTTTCCTTTCTCCATGAAGAGTTGGTTAAAGTGATGTCGGCAATAGAGAACGCTATCTAA
GGAGGCGTAGGAGGAGTGAGTCAGCGACGGGAAACGACAATCTGATCCCCGGGTACCCTG
TCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGG
ATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACG
TGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGG
   
Sequence Name 41-K033040-022-537-H11C-3144
GenBank Accession LN503979 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K033040-022-537-H11C-3144
ATCCACTATCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGTCGCTGACTCACTCCTCCTACGCCTCCT
TAGATAGCGTTCTCTATTGCCGACATCACTTTAACCAACTCTTCATGGAGAAAGGAAACT
ACGCTCACGTCCTCCAAGCCGCCAATCACCGTCGCACAGCCTCAGGCAACACTCTTCCAC
CGGAACCTACCGAAGACGTCGCCGTGGAGGCCAAGGAAGAGAATGGCGTTTCAGAGTCTT
GAATATCTCTTACATGAGTGTTTTTGAGTGAATGATTGAATGANGGTTGTTG

Last Updated on 06.12.2018 16:11