Sequences from confirmation amplicon


Confirmation sequences for line 561E02 and hit At5g55650 are shown.
Line ID 561E02
Locus At5g55650 [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22536385
Primers used for PCR NG40 - 8474, show primer details
   
Sequence Name 01-K102362-0022-561-E02C-8409
GenBank Accession LN486519 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined)
>01-K102362-0022-561-E02C-8409
TTGAATAATCCTGTAGCATTTTACTCGGTGTTGCTTTCTTTCACAGCTGTCACTGCTGTT
TTTTCTTGTAACCCTAATTTCATCTGTAGCTTTCCTCTGTTTCTTGAACTCTCAAGGAGT
CTCCTCTCTCACAATAACCATGGCAATCTTGTTCGTCTCCTCTTCTGTTTTCTTGTTACG
AATCTTCAAGGAAAAGACAAGACTATGTTTGGCTGAGGGGTCCCAAGGAGAAGGCTACGA
TTATGAAGATGATGATGATGATGATGGTCTCATTGAGATTGATCTTGTGAGCGAAGAAGC
AGAGACTATAGAGGCAATGAGAAACATTAATCAAAGTCGACTGAGAATTAGGATTGAAGA
AGAACAAGAAGAAGATATTGATGATGTGAATATGGAAGAAGATAATCTTATTGAGATTGA
TATTTCCATTGGATCCATTAAAAGACGAATGTAATTTAGTTTAATAGATCTCATGATCAC
ACCATCGTGTAATCCGATTTTTTCATTTCAAATGTACAAAGGAAATCCAACATTTTAATA
TATTATTGTTCTAGATATTTGGTGATTAGGGTTTAGGGTTTTAGGAGATATATATCAATC
CCTTTTTATAGATGCATATATATATATATATATATATATATATATATATATAGATATATA
TATGTGTGGGAGTTCTTTTGTATATAGAGTGTGTAATAGAGTGTGGACAAAACACAAAAG
AGTGTGAAATAT
   
Sequence Name 01-K102363-0022-561-E02C-NG40
GenBank Accession LN486520 [GenBank]
Sequencing primer gene specific primer, NG40: 5'-ATAAGTTAGCGGAGTTTGGGGTAT-3'
Sequence (A. th genome BLAST matches underlined)
>01-K102363-0022-561-E02C-NG40
GTTTTGTTTACAAACCTTTCAGGAATCTATTACACAATCTTTTGTCTTTTGTCAACAATC
TATTACACAATCTTAATGCAAAAGAACTACCACAGTTAATTAGTACTATATATATATATA
TATATATATATATATATATATATGTATCTATAAGGGGGGGGGGGTGTGTGT

Last Updated on 23.10.2014 13:25