Sequences from confirmation amplicon


Confirmation sequences for line 561F08 and hit At5g11510 are shown.
Line ID 561F08
Locus At5g11510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3681752
Primers used for PCR RS16 - 8474, show primer details
   
Sequence Name 63-K026231-022-561-F08C-8409
GenBank Accession LN510891 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined)
>63-K026231-022-561-F08C-8409
CTCCCGGACATGAAGCCATTTTTAATTCCATTATCCGACCTGTTGATAACATAGCAGAAA
ACTATATTGCTAGAACAAATAGAAGAATACCTTTAAGATTGAATAAATATGTACGATGAT
TTTTCAAATAACAATAGAAATGAGTACCTTCCAGGCAAGAACTTTGTTAACTCAGCCCAT
CTATTACCATATATTTGATGAGCACGAATCAGAAGCAGCTCTTCTTCTTGAGTCCAAGCT
TCTTTGTTTATGGCAGGATTGAGATGATTGTGCCACCTATATCAGTAACAGCCTTCACAG
TTAAAATCTTGAAAAGAGGACAAAAACTATATAGTGCTAGTAAAACACTGTTGTTAGAGA
AATAGCTGTCTCTATAAGCATACCTCTCTCTACATTGCTTTCCAATACGGCCAGGTAAAA
ATCGAGCAATAGTGGACCACTTCTTCGGCCCATATTTTTCGATAAACTG
   
Sequence Name 64-K026231-022-561-F08C-RS16
GenBank Accession LN511109 [GenBank]
Sequencing primer gene specific primer, RS16: 5'-TTCAGTTAATCGAAAAATATGGGC-3'
Sequence (A. th genome BLAST matches underlined)
>64-K026231-022-561-F08C-RS16
CGATTTTTACCTGGCCGTATTGGAAAGCAATGTAGAGAGAGGTATGCTTATAGAGACAGC
TATTTCTCTAACAACAGTGTTTTACTAGCACTATATAGTTTTTGTCCTCTTTTCAAGATT
TTAACTGTGAAGGCTGTTACTGATATAGGTGGCACAATCATCTCAATCCTGCCATAAACA
AAGAAGCTTGGACTCAAGAAGAAGAGCTGCTTCTGATTCGTGCTCATCAAATATATGGTA
ATAGATGGGCTGAGTTAACAAAGTTCTTGCCTGGAAGGTACTCATTTCTATTGTTATTTG
AAAAATCATCGTACATATTTATTCAATCTTAAAGGTATTCTTCTATTTGTTCTAGCAATA
TAGTTTTCTGCTATGTTATCAACAGGTCGGATAATGGAATTAAAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAG
TACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACA
AATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 07.07.2016 14:16