Sequences from confirmation amplicon


Confirmation sequences for line 600E10 and hit At5g65060 are shown.
Line ID 600E10
Locus At5g65060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25991191
Primers used for PCR KF26 - 8474, show primer details
   
Sequence Name 57-K103224-0022-600-E10C-KF26
GenBank Accession LN509384 [GenBank]
Sequencing primer gene specific primer, KF26: 5'-ATGCAACACAGTTTTGAGTGAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K103224-0022-600-E10C-KF26
CAAGAAGTATACTCTTAAAATCTAGATTTCTTGTTAATTTTTGTTAGCTGTAGGAAAATA
ATGTAAGAAAGTTTCTTACAGGTGGGGAAGAAGACGTTTCTGGTTATAGAAGGTGACAGA
GGAATGTCACGGGAAAATGGCTCCGGCAACAAAGTACCGGAGACTCTTTCGCTGCTCAAG
TAATCACCATCATCAACGGCTGAGCTTTCACCATAAACTTACTCACAGCCTGATTCAGAA
GCTTTTACAAAATTGTAAATTATAAAAAGCTGCATAATAATCTCAACCTTTTTATCTTCC
TCGCGCCAATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATNTCCGCACGATTATA
   
Sequence Name 25-K103225-0022-600-E10C-8409
GenBank Accession LN497396 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K103225-0022-600-E10C-8409
TCGGACTGAGCCTTTACTTGGCGCGAGGAAGAAAAAAGGTTGAGATTATTATGCAGCTTT
TTATAATTTACAATTTTGTAAAAGCTTCTGAATCAGGCTGTGAGTAAGTTTATGGTGAAA
GCTCAGCCGTTGATGATGGTGATTACTTGAGCAGCGAAAGAGTCTCCGGTACTTTGTTGC
CGGAGCCATTTTCCCGTGACATTCCTCTGTCACCTTCTATAACCAGAAACGTCTTCTTCC
CCACCTGTAAGAAACTTTCTTACATTATTTTCCTACAGCTAACAAAAATTAACAAGAAAT
CTAGATTTTTAAGAGTATACTTTTCTTTTGCTTAAAACTAAATTGTGAGTTTCACTCAAA
ACGNTGTTGCA

Last Updated on 06.12.2018 16:11