Sequences from confirmation amplicon


Confirmation sequences for line 606B04 and hit At3g62090 are shown.
Line ID 606B04
Locus At3g62090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22989158
Primers used for PCR TN06 - 8474, show primer details
   
Sequence Name 04-K103793-0022-606-B04C-TN06
GenBank Accession LN487888 [GenBank]
Sequencing primer gene specific primer, TN06: 5'-TTTGTATGCTATGCCTCTTGTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K103793-0022-606-B04C-TN06
GTCAGCAGCTTGTTTCATAAACAGAGAGGAACGTCGAGTTATGATTTTTATCACTCCGAT
AGTGTTCGATAATTTATTAATGTTTCTAACATGCAGTCTTATTCATTCGAAGGAAACTAG
TACTCGTGATTATTATTGTTATTATTATAGCTCCTTTCCTATATCTATATGTTACATGAA
CTAGAAAAGCCGGCACTAAAAAAGAGAACCTTGAGGAGATCCTTCCATGGTAGTTGTTCT
GATGTCGTTGTAGTGCTTTTGTGGATCTGTATGGTTCACCTTGAGATGGCTCTAGTGTTC
TCACGACTAACTCATGAAGAAGAAGACCGTGAATCCGCAGGTAACAATCATCACTGTTTA
CTTCATCTGCTCACGGGTTTCCAGATTTTTACCAGGGAGAATTCCTTCATCTGTTAGTTT
TCCTTGATTTCTTGTGTAAGGTTGCACCATCTAAATTTGACAAAGAGTTGGGATTTGGTA
TTTGGTTAGGGAAAGCAGCGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAAATGTCCGCAGCGTTATT
   
Sequence Name 12-K103794-0022-606-B04C-8409
GenBank Accession LN491546 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K103794-0022-606-B04C-8409
AATCCACTCTTTGTCAATTTAGATGGTGCAACCTTACACAAGAAATCAAGGAAAACTAAC
AGATGAAGGAATTCTCCCTGGTAAAAATCTGGAAACCCGTGAGCAGATGAAGTAAACAGT
GATGATTGTTACCTGCGGATTCACGGTCTTCTTCTTCATGAGTTAGTCGTGAGAACACTA
GAGCCATCTCAAGGTGAACCATACAGATCCACAAAAGCACTACAACGACATCAGAACAAC
TACCATGGAAGGATCTCCTCAAGGTTCTCTTTTTTAGTGCCGGCTTTTCTAGTTCATGTA
ACATATAGATATAGGAAAGGAGCTATAATAATAACAATAATAATCACGAGTACTAGTTTC
CTTCGAATGAATAAGACTGCATGTTAGAAACATTAATAAATTATCGAACACTATCGGAGT
GATAAAAATCATAACTCGACGTTCCTCTCTGTTTATGAAACAAGCTGCTGACTTTATACA
ATGGAGTGAAGCTATTGTTTCTAGAATCCAAACAAGAGGCATAGCATACAA

Last Updated on 06.12.2018 16:11