Sequences from confirmation amplicon


Confirmation sequences for line 609E06 and hit At4g10490 are shown.
Line ID 609E06
Locus At4g10490 [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6484440
Primers used for PCR MZ40 - 8474, show primer details
   
Sequence Name 54-K024138-022-609-E06C-MZ40
GenBank Accession LN508351 [GenBank]
Sequencing primer gene specific primer, MZ40: 5'-AAGTGCACAAACAATCCACTTTTA-3'
Sequence (A. th genome BLAST matches underlined)
>54-K024138-022-609-E06C-MZ40
CTATACTCTGGTTTCTTGAGTAACACTCTTATCTTTTCTTGATTTTACACTAGGGTTTTG
CAATACTGTTTTTCTTTCTTTCATGATTTTACACTAGATTTGTTTCCTCTGTTCCAGATC
AAGAACCATGGAGTACCAGAAGAAACGATCAAAAAAATGATGAATGCGGCAAGAGAGTTT
TTCCGGCAATCAGAGAGCGAAAGAGTGAAGCATTACTCAGCAGATACCAAAAAGACAACG
AGACTCTCCACAAGTTTCAACGTTTCCAAAGAGAAAGTCTCTAACTGGAGACTATATGGG
CCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA
CGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCG
GAAATCTTTATTTCGACGTGTCTACATTCACG
   
Sequence Name 55-K024138-022-609-E06C-8409
GenBank Accession LN508684 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined)
>55-K024138-022-609-E06C-8409
CCCATATAGTCTCCAGTTAGAGACTTTCTCTTTGGAAACGTTGAAACTTGTGGAGAGTCT
CGTTGTCTTTTTGGTATCTGCTGAGTAATGCTTCACTCTTTCGCTCTCTGATTGCCGGAA
AAACTCTCTTGCCGCATTCATCATTTTTTTGATCGTTTCTTCTGGTACTCCATGGTTCTT
GATCTGGAACAGAGGAAACAAATCTAGTGTAAAATCATGAAAGAAAGAAAAACAGTATTG
CAAAACCCTAGTGTAAAATCAAGAAAAGATAAGAGTGTTACTCAAGAAACCAGAGTATAG
GAGATTAAAAGTGGATTGTTTGTGCACT

Last Updated on 23.10.2014 13:25