Sequences from confirmation amplicon


Confirmation sequences for line 611A02 and hit At5g18450 are shown.
Line ID 611A02
Locus At5g18450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6117157
Primers used for PCR NG07 - 8474, show primer details
   
Sequence Name 33-K102366-0022-611-A02C-8409
GenBank Accession LN500941 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K102366-0022-611-A02C-8409
ATTGAATATATCCTGTGTGATGTATATCATGTATATGTTTTACACGTACACACTCATATT
TACATTTGCCACGTAAGAATACGTATACTCCTGACGTGTCCTGTATATATATACACACGC
ACACTTCAATTTTTCATTCACTAAACCAGATTTAATTTGAGACTCTCAAAGAAACACCAT
GGAAGAAGAGCAACCTCCGGCCAAGAAACGAAACATGGGGAGATCTAGAAAAGGTTGCAT
GAAAGGTAAAGGCGGTCCAGAGAACGCCACGTGTACTTTCCGTGGAGTTAGGCAACGGAC
TTGGGGTAAATGGGTGGCTGAGATCCGTGAGCCTAACCGTGGGACTCGTCTCTGGCTCGG
CACGTTTAATACCTCGGTCGAGGCCGCCATGGCTTACGATGAAGCCGCTAAGAAACTCTA
TGGACACGAGGCTAAACTCAACTTGGTGCACCCACAACAACAACAACAAGTAGTAGTGAA
CAGAAACTTGTCTTTTTCTGGCCACGGGTCGGGTTCTTGGGCTTATAATAAGAAGCTCGA
TATGGTTCATGGGTTGGACCTTGGTCTCGGCCAGGCAAGTTGTTCACGAGGTTCTTGCTC
AGAGAGATCGAGTTTTCTACAAGAAGATGATGATCATAGTCATAATCGA
   
Sequence Name 33-K102367-0022-611-A02C-NG07
GenBank Accession LN500942 [GenBank]
Sequencing primer gene specific primer, NG07: 5'-CTTGAAGACGAACATCGATTATGA-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K102367-0022-611-A02C-NG07
CTCTGAGCAGAACCTCGTGAACAACTTGCCTGGCCGAGACCAAGGTCCAACCCATGAACC
ATATCGAGCTTCTTATTATAAGCCCAAGAACCCGACCCGTGGCCAGAAAAAGACAAGTTT
CTGTTCACTACTACTTGTTGTTGTTGTTGTGGGTGCACCAAGTTGAGTTTAGCCTCGTGT
CCATAGAGTTTCTTAGCGGCTTCATCGTAAGCCATGGCGGCCTCGACCGAGGTATTAAAC
GTGCCGAGCCAGAGACGAGTCCCACGGTTAGGCTCACGGATCTCAGCCACCCATTTACCC
CAAGTCCGTTGCCTAACTCCACGGAAAGTACACGTGGCGTTCTCTGGACCGCCTTTACCT
TTCATGCAACCTTTTCTAGATCTCCCCATGTTTCGTTTCTTGGCCGGAGGTTGCTCTTCT
TCCATGGTGTTTCTTTGAGAGTCTCAAATTAAATCTGGTTTAGTGAATGAAAAATTGAAG
TGTGCGTGTGTATATATATACAGGACACGTCAGGAGTATACGTATTCTTACGTGGCAAAT
GTAAATATGAGTGTGTACGTGTAAAACATATACATGATATACATCACACAGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA

Last Updated on 06.12.2018 16:11