Sequences from confirmation amplicon


Confirmation sequences for line 626E06 and hit At5g49770 are shown.
Line ID 626E06
Locus At5g49770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20224488
Primers used for PCR DW05 - 8474, show primer details
   
Sequence Name 45-K026694-022-626-E06C-8409
GenBank Accession LN505399 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K026694-022-626-E06C-8409
TTTGGAAAAAAATTTTTCTCCACCTTCCACCCCATCCAATTTTTAAACTAGGCCAAGGTT
TTCGGGAAGGTTCCCGGGAAATTGGTTTCCATCAAACAGCCTATTGATAAAACAAGAACA
AAAATGCAATCATCACTTTGTTAGTTTCTAATATCAACTAGTGAAAACTCTCAGCTTTTG
CATGTACTATGAAACTTACACATGTAGCAAAGTCATTTCTGAGCTAAAGAGCTTCTCTGG
GATTTCGCCTGAAAG
   
Sequence Name 46-K026694-022-626-E06C-DW05
GenBank Accession LN505743 [GenBank]
Sequencing primer gene specific primer, DW05: 5'-TAATAACAAGCTTTCAGGCGAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K026694-022-626-E06C-DW05
CTCTTTAGCTCAGAAATGACTTTGCTACATGTGTAAGTTTCATAGTACATGCAAAAGCTG
AGAGTTTTCACTAGTTGATATTAGAAACTAACAAAGTGATGATTGCATTTTTGTTCTTGT
TTTATCAATAGGCTGTTTGATGGAAACCAATTCACCGGTAGCATCCCAGAGAGCCTCGGC
CTAGTTCAAAATTTGACTGTGTTGTAAGTAGTAGAAATATATTTTCCAAACTGTATGTTC
ACAAAATTTCTTTAATCTTATAAGATATTTGCTTTGTTACAGACGTCTTGATAGGAATAG
ACTAAGTGGAGACATTCCTTCAAGTCTTAATAATCTCACAAATCTTCAAGAACTGTAAGT
GAGCAAATAAAGATTTGTTCTGTTTTCTTAAGATATATAAATGAAAAGATGTAAAAGATC
TTGTAGTTGAATCCATTTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AGCGTTATTATAAAATGAAAGTACTTTTTGATAAAACGACAAATTACGATCCGTCGTATT
TATAGGCG

Last Updated on 06.12.2018 16:11