Sequences from confirmation amplicon


Confirmation sequences for line 659C02 and hit At5g64380 are shown.
Line ID 659C02
Locus At5g64380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25741300
Primers used for PCR MA64 - 8474, show primer details
   
Sequence Name 69-K102435-0022-659-C02C-8409
GenBank Accession LN512169 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined)
>69-K102435-0022-659-C02C-8409
TTACAATTGAATATATCCTGATGGCGAGACGCCATATCGCGACGGTTAATACTGGCGGTC
GGAGCAGAGAGAACAGAAGCTGCGATTTGTCTTAGCGGGTAAAGATTGAGTCTTGGCGGG
GAAATTGGAGTTGGGTACCGAGTTAAGGTAAGAGATTGCATTATTTTCTAGAATTTAAAC
CCGAGTACGCTTCAAAACGGATAACGAATAGAAGATGACGAGGAGTAGAAAACCCCACAG
TTCATAGTATCTGATTACGGTTATTTTTGGAATTGGATTCCGGTTCAATTATAAACCGGT
TCGATTATAAAAGCAAAGGAGCTGCCTTTTGGCATCAACATATATTCAGCTACAAACAGA
TTTTCAGTTACCGTTACATTTACACAAATGATTGAACATTGATTATTGGACTTTTCTTCT
TTGAAGATTCACAGAGTGAAATTTTCTTCTTATGTAGAATTCTTGTGGAGCAATGGGTCA
GAGACGACTTGAGGCTTGAAGTCTGGCTTTATGTACTTTGCCAAAAGATCGGCATAGACT
TCGTATCTTGCAGTCATTCTAAATCCCCACTTG
   
Sequence Name 69-K102436-0022-659-C02C-MA64
GenBank Accession LN512170 [GenBank]
Sequencing primer gene specific primer, MA64: 5'-ACAAGTGGGGATTTAGAATGACTG-3'
Sequence (A. th genome BLAST matches underlined)
>69-K102436-0022-659-C02C-MA64
TTTGGCAAGTAATAAAGCCAGACTTCAAGCCTCAAGTCGTCTCTGACCCATTGCTCCACA
AGAATTCTACATAAGAAGAAAATTTCACTCTGTGAATCTTCAAAGAAGAAAAGTCCAATA
ATCAATGTTCAATCATTTGTGTAAATGTAACGGTAACTGAAAATCTGTTTGTAGCTGAAT
ATATGTTGATGCCAAAAGGCAGCTCCTTTGCTTTTATAATCGAACCGGTTTATAATTGAA
CCGGAATCCAATTCCAAAAATAACCGTAATCAGATACTATGAACTGTGGGGTTTTCTACT
CCTCGTCATCTTCTATTCGTTATCCGTTTTGAAGCGTACTCGGGTTTAAATTCTAGAAAA
TAATGCAATCTCTTACCTTAACTCGGTACCCAACTCCAATTTCCCCGCCAAGACTCAATC
TTTACCCGCTAAGACAAATCGCAGCTTCTGTTCTCTCTGCTCCGACCGCCAGTATTAACC
GTCGCGATATGGCGTCTCGCCATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTA
CATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAA
TTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 07.07.2016 14:16