Sequences from confirmation amplicon


Confirmation sequences for line 742G06 and hit At2g47430 are shown.
Line ID 742G06
Locus At2g47430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19460544
Primers used for PCR CM49 - 8474, show primer details
   
Sequence Name 60-K104191-0022-742-G06C-CM49
GenBank Accession LN510247 [GenBank]
Sequencing primer gene specific primer, CM49: 5'-ATCGAATTCCATAAGAAACAATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K104191-0022-742-G06C-CM49
GTGTTGAGTGGAGAACTGGTAAGGGATACCTATGGAGATGCGTAAGTCGGTATTTGAAAA
CTATGTTCAAGTAAGAGAAACAGCTCAAGGACACCAAGGAACTGGTTTAGGGCTCGGGAT
TGTGCAGTCTTTGGTAAGCTACTAAAACAGAACACGTTCTTTCTCTTCACAAGTAAATCT
AGATGGTTTTCATTTGTGGTCTATTATTATAGGTAAGATTAATGGGAGGGGAGATAAGAA
TCACCGACAAGGCCATGGGAGAGAAAGGAACATGTTTCCAATTCAATGTTTTATTGACAA
CATTAGAGTCTCCTCCAGTGAGTGACATGAAAGTGAGACAGGAGATCGAAGCAGGAGGCG
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCACGATTATT
   
Sequence Name 72-K104192-0022-742-G06C-8409
GenBank Accession LN512718 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K104192-0022-742-G06C-8409
CCATTTACAATTGCGCCTCCTGCTTCGATCTCCTGTCTCACTTTCATGTCACTCACTGGA
GGAGACTCTAATGTTGTCAATAAAACATTGAATTGGAAACATGTTCCTTTCTCTCCCATG
GCCTTGTCGGTGATTCTTATCTCCCCTCCCATTAATCTTACCTATAATAATAGACCACAA
ATGAAAACCATCTAGATTTACTTGTGAAGAGAAAGAACGTGTTCTGTTTTAGTAGCTTAC
CAAAGACTGCACAATCCCGAGCCCTAAACCAGTTCCTTGGTGTCCTTGAGCTGTTTCTCT
TACTTGAACATAGTTTTCAAATACCGACTTACGCATCTCCATAGGTATCCCTTTACCAGT
ATCATCCACTTCAAACACAAACTCCATCGTGTTTGCATTGTTTCTTATGGAATTCG

Last Updated on 06.12.2018 16:11