Sequences from confirmation amplicon


Confirmation sequences for line 757A11 and hit At1g02660 are shown.
Line ID 757A11
Locus At1g02660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:572181
Primers used for PCR 13ci - 8474, show primer details
   
Sequence Name 61-K107936-0022-757-A11C-13ci
GenBank Accession LN510539 [GenBank]
Sequencing primer gene specific primer, 13ci: 5'-GTATCTGAAAGCGGTTAGGAGTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K107936-0022-757-A11C-13ci
TAGGAAGCAAAAGGGAGCATCGCCGGAGTCTTTGGTGGCCAATTCTGGTGGCTAGAGAAA
GTGGAAGCTCAGGGATTGCGGTCAGTAACGGCCAAATCAACGGTCAGGATTTCTCCGGGA
TGATGCAGACAGGAAGAAAGTCGTTGCAGAGGTTTAGCCGCCTTGTGGCGTCTCAACATA
TGCCGTTGATCGTTGTTATGTTGTTTCCGGTTAAGTTGTTGTTCCTTGGAGCTTTCAACG
TCTTTAGTTTCCGTTGAAAAGTTTCTTTTGGCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTA
   
Sequence Name 45-K107935-0022-757-A11C-8409
GenBank Accession LN505551 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K107935-0022-757-A11C-8409
CGCAAGAACTTTTCACGGAAACTAAAGACGTTGAAAGCTCCAAGGAACAACAACTTAACC
GGAAACAACATAACAACGATCAACGGCATATGTTGAGACGCCACAAGGCGGCTAAACCTC
TGCAACGACTTTCTTCCTGTCTGCATCATCCCGGAGAAATCCTGACCGTTGATTTGGCCG
TTACTGACCGCAATCCCTGAGCTTCCACTTTCTCTAGCCACCAGAATTGGCCACCAAAGA
CTCCGGCGATGCTCCCTTTTTGCTCTCCTTATCTGATTCACTTCCTTTCTTATTACACTC
CTAACCGCTTTCAGATA

Last Updated on 06.12.2018 16:11