Genotyping and primer details


Genotyping and primer details for 757A11 - At1g02660
Line ID 757A11
Locus At1g02660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:572181 of line 757A11 Chr1:572181
   
Insertion allele amplicon 8474 - 13ci
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13ci: 5'-GTATCTGAAAGCGGTTAGGAGTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:572493
   
wt amplicon (over InsPos) 13cr - 13ci
wt amplicon start Chr1:571617
wt amplicon end Chr1:572493
wt amplicon size 877 bp
Locus specific primer 13ci: 5'-GTATCTGAAAGCGGTTAGGAGTGT-3'
2nd locus specific primer 13cr: 5'-CAGTTTTCAAAATAATTTCGCCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11