Sequences from confirmation amplicon


Confirmation sequences for line 773C12 and hit At2g34340 are shown.
Line ID 773C12
Locus At2g34340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14489074
Primers used for PCR 04ux - 8474, show primer details
   
Sequence Name 92-K117671-0022-773-C12C-04ux
GenBank Accession LN515211 [GenBank]
Sequencing primer gene specific primer, 04ux: 5'-TGTCTCTCACAATCAATGACAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>92-K117671-0022-773-C12C-04ux
TCTGCACTAATAAATCCTAACTCAAATATATTCATCACACTTTCATAATTCCAATTAAGT
ACCCCCAAAAATACACTTGACAAGCCTTGCATTTTAAATCCTAACACAAGAATTATTGTT
ATTCTTCTCATAATTAACCCGAAATTTCCTTGTTTCCTATTACAATTTAGGAAAAACAAA
AGAATATGGAAAAACAAAAACTAAGGAGAAAGCTTTGTTTCACATTTTCCCCACCCTTTA
CTTAAATAAACAACACGGGGATCCACTAGCAATTTTCCGTAATATGCAAAAAAACAAAAG
AGTTGGGAAAAGATGATGAAGTATGGCAAACCGGTAACGGTTCAAGCCTCCAAAAAACCG
GTTAACCTAAGAACCGAATTCCGATGGATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTTATTATAAA
   
Sequence Name 44-K117670-0022-773-C12C-8409
GenBank Accession LN505321 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K117670-0022-773-C12C-8409
ATTTACATTGAAATATCCATCGGAATTCGGTTCTTAGGTTAACCNGGTTTTTTGGAGGCT
TGAACCGTTACCGGTTTGCCATACTTCATCATCTTTTCCCAACTCTTTTGTTTTTTTGCA
TATTACGGAAAATTGCTAGTGGATCCCCGTGTTGTTTATTTAAGTAAAGGGTGGGGAAAA
TGTGAAACAAAGCTTTCTCCTTAGTTTTTGTTTTTCCATATTCTTTTGTTTTTCCTAAAT
TGTAATAGGAAACAAGGAAATTTCGGGTTAATTATGAGAAGAATAACAATAATTCTTGTG
TTAGGATTTAAAATGCAAGGCTTGTCAAGTGTATTTTTGGGGGTACTTAATTGGAATTAT
GAAAGTGTGATGAATATATTTGAGTTAGGATTTATGTAGTGCAGATGTGGACAAAAGCCA
AACAGAAACAGCTGTTCTGTTGTCATTGATTGGGAGAGACA

Last Updated on 06.12.2018 16:11