Sequences from confirmation amplicon


Confirmation sequences for line 787G12 and hit At5g46370 are shown.
Line ID 787G12
Locus At5g46370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18811819
Primers used for PCR MJ69 - 8474, show primer details
   
Sequence Name 18-K029635-022-787-G12C-8409
GenBank Accession LN494175 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K029635-022-787-G12C-8409
AGCCATTTACAATTGACCATCAGACAGAAAAAGCTTCTTCCTTTATCTCCACCATTATCG
GAAAACGACCCTAGTTTCAAAAATCTCTCAACTTTCCAGTTTCCCGGCGAAAACCCAGAT
GGCTAACGACGGTAACGGCGATAACAACGATGACCCATTACGCCAATACTTGATGAACCC
AAGAATCAACCCACCACCACCGTCTCTGTTAACATTACCGGAGAACAATGACGTCACAAT
TCCGATGCCAATAACTCCATTGGAACTTAAAAACCGATTAATCTTCGGTTCATTCGTTCG
TTCTAGAAAAGAATCATCTCTTCCCATCGATGCTCTCTCTCAAAACCCTTC
   
Sequence Name 19-K029635-022-787-G12C-MJ69
GenBank Accession LN494632 [GenBank]
Sequencing primer gene specific primer, MJ69: 5'-TAGAAGGGTTTTGAGAGAGAGCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K029635-022-787-G12C-MJ63
AATGACCGAAGATTAATCGGTTTTTAAGTTCCAATGGAGTTATTGGCATCGGAATTGTGA
CGTCATTGTTCTCCGGTAATGTTAACAGAGACGGTGGTGGTGGGTTGATTCTTGGGTTCA
TCAAGTATTGGCGTAATGGGTCATCGTTGTTATCGCCGTTACCGTCGTTAGCCATCTGGG
TTTTCGCCGGGAAACTGGAAAGTTGAGAGATTTTTGAAACTAGGGTCGTTTTCCGATAAT
GGTGGAGATAAAGGAAGAAGCTTTTTCTGTCTGATGGTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAA
CAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAAATG

Last Updated on 06.12.2018 16:11