Sequences from confirmation amplicon


Confirmation sequences for line 815E02 and hit At5g65060 are shown.
Line ID 815E02
Locus At5g65060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25988141
Primers used for PCR KF28 - 8474, show primer details
   
Sequence Name 66-K103226-0022-815-E02C-KF28
GenBank Accession LN511582 [GenBank]
Sequencing primer gene specific primer, KF28: 5'-TACACACCGAAAAGGGTAAAAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K103226-0022-815-E02C-KF28
ATGTGCATGTGGGGATGCATCAATGTGGAAAATTATCCAGCGTTACAATCAACCTTCAAA
ATCATCTATAGACCTTTAATGTACTTAATCGGCAAATCCATTGGAGAGAAATTAAAAACA
ATCAGTGTAGTCTAGACTACAATACATTCGGGATCAGTAATTCCACAATGTAATTTAAAC
GAAAGACTCTTGGTAAATGTTACCATTAACCCCAATTACTCTATCAACAAATCCACGGGA
GGTGCATTGCAACATTGGATACAAACCAAATCTCAAAATTTAACAAATAGACNGCAATCT
ACATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATNTCC
GCA
   
Sequence Name 69-K103227-0022-815-E02C-8409
GenBank Accession LN512178 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K103227-0022-815-E02C-8409
TTCGGACATGAGCCATTTACTGTAGATTGCTGTCTATTTGTTAAATTTTGAGATTTGGTT
TGTATCCAATGTTGCAATGCACCTCCCGTGGATTTGTTGATAGAGTAATTGGGGTTAATG
GTAACATTTACCAAGAGTCTTTCGTTTAAATTACATTGTGGAATTACTGATCCCGAATGT
ATTGTAGTCTAGACTACACTGATTGTTTTTAATTTCTCTCCAATGGATTTGCCGATTAAG
TACATTAAAGGTCTATAGATGATTTTGAAGGTTGATTGTAACGCTTGGATAATTTTCCAC
ATTGATGCATCCCCACATGCTACATTACTTATCTAATCGGACATAGATTTTTACCCTTTT
CGGTGTGT

Last Updated on 06.12.2018 16:11