Sequences from confirmation amplicon


Confirmation sequences for line 859F04 and hit At2g46660 are shown.
Line ID 859F04
Locus At2g46660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19154212
Primers used for PCR IF74 - 8474, show primer details
   
Sequence Name 58-K101502-0022-859-F04C-8409
GenBank Accession LN509657 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K101502-0022-859-F04C-8409
GCCTTTATTCGAGGAACAGACACAGTTGCGGTCTTAATCGAGTGGATCCTCGCTAGGATG
GTCCTTCATCCAGATATGCAATCAACGGTACAAAACGAGCTGGATCAAGTAGTCGGGAAA
TCAAGAGCCCTAGATGAATCTGACTTGGCTTCACTTCCATATCTAACGGCTGTGGTGAAA
GAAGTATTGAGGCTTCATCCTCCAGGCCCACTTCTATCATGGGCCCGTTTGGCCATAACA
GACACGATCGTTGATGGTCGTCTTGTTCCGGCAGGGACCACAGCAATGGTGAACATGTGG
GCCGTATCGCATGATCCACACGTGTGGGTTGATCCTTTGGAGTTTAAACCTGAGAGGTTC
GTGGCAAAAGAAGGTGAGGTGGAGTTTTCGGTTCTTGGGTCGGATTTGAGACTTGCACCT
TTCGGGTCGGGTCGTCGGAATTGCCCCGGGAAGAATCTTGGTTTTACTACCGTTATGTTT
TGGACGGCGATGATGTTACATGAGTTTGAATGGGG
   
Sequence Name 58-K101506-0022-859-F04C-IF74
GenBank Accession LN509658 [GenBank]
Sequencing primer gene specific primer, IF74: 5'-AATTTAGCAGGAAGAGGATTAGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K101506-0022-859-F04C-IF74
ATAGTCAACGCCGTTCCATCGGACGGTCCCCATTCAAACTCATGTAACATCATCGCCGTC
CAAAACATAACGGTAGTAAAACCAAGATTCTTCCCGGGGCAAATCCGACGACCCGACCCG
AAAGGTGCAAGTCTCAAATCCGACCCAAGAACCGAAAACTCCACCTCACCTTCTTTTGCC
ACGAACCTCTCAGGTTTAAACTCCAAAGGATCAACCCACACGTGTGGATCATGCGATACG
GCCCACATGTTCACCATTGCTGTGGTCCCTGCCGGAACAAGACGACCATCAACGATCGTG
TCTGTTATGGCCAAACGGGCCCATGATAGAAGTGGGCCTGGAGGATGAAGCCTCAATACT
TCTTTCACCACAGCCGTTAGATATGGAAGTGAAGCCNAGTCAGATTCATCTNGGGCTCTG
GATTTCCCGACTACTTGATCCAGCTCGTTTTGTACCGTTGATTGCATATCTGGATGAAGG
ACCATCCTAGCG

Last Updated on 06.12.2018 16:11