Sequences from confirmation amplicon


Confirmation sequences for line 928C10 and hit At1g49760 are shown.
Line ID 928C10
Locus At1g49760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18419565
Primers used for PCR 50kf - 8474, show primer details
   
Sequence Name 88-K113549-0022-928-C10C-50kf
GenBank Accession LN514884 [GenBank]
Sequencing primer gene specific primer, 50kf: 5'-TCTGTTTTCGGAAGCAAGAATAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K113549-0022-928-C10C-50kf
ATTGATCATTCGAAGCTTTTAAACCAAATTTTATACCCAAAAGTCGAAACTCTAGACAAA
AGCCAAACAATACCCTATCAAATGAGCAGTGTAGGTAAAAAAAGGTGAGAGTAACGATAC
AAAACTTCCCAAAGTATTAAGAAAATCAAAAACACAGAAGGAAAATGTTAAAACTCTCGC
CCAAAACAAAAACTCAAAAATCCAAAACTTTCAAGAACAACTCTGTTTCTGATTCTCCAA
AAGAACAAAATAAACACTTTTACAGTGTATATAAACTGAGCTCTTTCAGATATAACTTAG
AACTCTCAAGGTACGATGTTGTCTCCAAGAGACAGAGAAGCTAACTGATCAGCTGCACCA
CCCGCTTGCTGCTGTGCTACACTCCTCAGAACATCCATTGCTTCTGTAACTTTAGCTTTA
AGTGCCTCTGGTGATTCCAGCAAGTGAAGTACTTCGGTTTGGTCCATTTCAAGAAGCATT
CCCGTGACTTTAGCAATGAGTAAACGTTATACAGATGCTTCGGTTTGTGCAGGATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCACGGTTATT
   
Sequence Name 23-K113548w-0022-928-C10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K113548w-0022-928-C10C-8409
TCATTGAATTATCCTGCAAAACCGAAGCATCTGTATAACGTTTACTCATTGCTAAAGTCA
CGGGAATGCTTCTTGAAATGGACCAAACCGAAGTACTTCACTTGCTGGAATCACCAGAGG
CACTTAAAGCTAAAGTTACAGAAGCAATGGATGTTCTGAGGAGTGTAGCACAGCAGCAAG
CGGGTGGTGCAGCTGATCAGTTAGCTTCTCTGTCTCTTGGAGACAACATCGTACCTTGAG
AGTTCTAAGTTATATCTGAAAGAGCTCAGTTTATATACACTGTAAAAGTGTTTATTTTGT
TCTTTTGGAGAATCAGAAACAGAGTTGTTCTTGAAAGTTTTGGATTTTTGAGTTTTTGTT
TTGGGCGAGAGTTTTAACATTTTCCTTCTGTGTTTTTGATTTTCTTAATACTTTGGGAAG
TTTTGTATCGTTACTCTCACCTTTTTTTACCTACACTGCTCATTTGATAGGGTATTGTTT
GGCTTTTGTCTAGAGTTTCGACTTTTGGGTATAAAATTTGGTTTAAAAGCTTCGAATGAT
CAATGTAACAATAAGTTTACAGGGCTTTTCTAGGAAATTATTCTTGCTTCCGAAAACAGA
AAANCCCNCGTNNCTCTCCTCCTCCTCTTCCTTCTCNTCGT

Last Updated on 06.12.2018 16:11