Genotyping and primer details


Genotyping and primer details for 138H01 - At4g11230
Line ID 138H01
Locus At4g11230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:6845631 of line 138H01 Chr4:6845631
   
Insertion allele amplicon 8474 - FL15
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FL15: 5'-TCATGGATTGAGTTGAGTTGAGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:6846131
   
Insertion allele amplicon 3144 - ME47
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer ME47: 5'-AGGTCTAGTTGGTCGCTGAGATAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:6845114
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11