Genotyping and primer details


Genotyping and primer details for 010H02 - At1g78130
Line ID 010H02
Locus At1g78130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29401271 of line 010H02 Chr1:29401271
   
Insertion allele amplicon 8474 - GL04
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer GL04: 5'-ATCTGTGGCTATCTCTGTTGACCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29401565
   
wt amplicon (over InsPos) GL04 - FM64
wt amplicon start Chr1:29400746
wt amplicon end Chr1:29401565
wt amplicon size 820 bp
Locus specific primer FM64: 5'-GGAGAGTTGCTTTCCATATCGTAG-3'
2nd locus specific primer GL04: 5'-ATCTGTGGCTATCTCTGTTGACCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11