Genotyping and primer details


Genotyping and primer details for 052B10 - At3g16980
Line ID 052B10
Locus At3g16980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5795321 of line 052B10 Chr3:5795321
   
Insertion allele amplicon 8474 - 10eq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 10eq: 5'-TGAATACGGATTTGTCTCTGACTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5795824
   
wt amplicon (over InsPos) 10fo - 10eq
wt amplicon start Chr3:5794755
wt amplicon end Chr3:5795824
wt amplicon size 1070 bp
Locus specific primer 10eq: 5'-TGAATACGGATTTGTCTCTGACTG-3'
2nd locus specific primer 10fo: 5'-TGTTTTTGGGTCCTTTCTTAATTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11