Genotyping and primer details


Genotyping and primer details for 064B02 - At3g63070
Line ID 064B02
Locus At3g63070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:23307514 of line 064B02 Chr3:23307514
   
Insertion allele amplicon 8474 - 07sj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 07sj: 5'-CCATATTCATCCACCAGTATACCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:23308056
   
wt amplicon (over InsPos) 08tc - 07sj
wt amplicon start Chr3:23307107
wt amplicon end Chr3:23308056
wt amplicon size 950 bp
Locus specific primer 07sj: 5'-CCATATTCATCCACCAGTATACCC-3'
2nd locus specific primer 08tc: 5'-TTAATTAGAGGTGGAATCCAATCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11