Genotyping and primer details


Genotyping and primer details for 108H10 - At3g59570
Line ID 108H10
Locus At3g59570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22001881 of line 108H10 Chr3:22001881
   
Insertion allele amplicon 8474 - PC43
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PC43: 5'-ATTTAATTACTATTGCACCCGCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22002390
   
wt amplicon (over InsPos) 11tf - PC43
wt amplicon start Chr3:22001564
wt amplicon end Chr3:22002390
wt amplicon size 827 bp
Locus specific primer PC43: 5'-ATTTAATTACTATTGCACCCGCTG-3'
2nd locus specific primer 11tf: 5'-AAATGCAGTGAAAAACATCCTACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11