Genotyping and primer details


Genotyping and primer details for 127D05 - At3g14830
Line ID 127D05
Locus At3g14830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4984907 of line 127D05 Chr3:4984907
   
Insertion allele amplicon 3144 - 06vo
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 06vo: 5'-GGTAGAAGCTTCACATTCGAGTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4984437
   
wt amplicon (over InsPos) 48vx - 06vo
wt amplicon start Chr3:4984437
wt amplicon end Chr3:4985462
wt amplicon size 1026 bp
Locus specific primer 06vo: 5'-GGTAGAAGCTTCACATTCGAGTTC-3'
2nd locus specific primer 48vx: 5'-AGTTTTGCTTACAGCTACAGAGGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11