Genotyping and primer details


Genotyping and primer details for 132D09 - At3g27690
Line ID 132D09
Locus At3g27690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:10256103 of line 132D09 Chr3:10256103
   
Insertion allele amplicon 8474 - 09lu
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 09lu: 5'-ATATAGCTAATATGCTTTGCGCGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:10256604
   
wt amplicon (over InsPos) 07mo - 09lu
wt amplicon start Chr3:10255722
wt amplicon end Chr3:10256604
wt amplicon size 883 bp
Locus specific primer 09lu: 5'-ATATAGCTAATATGCTTTGCGCGT-3'
2nd locus specific primer 07mo: 5'-CAACACTATCCACTTGTTCGCTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11