Genotyping and primer details


Genotyping and primer details for 144C03 - At1g20600
Line ID 144C03
Locus At1g20600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7133619 of line 144C03 Chr1:7133619
   
Insertion allele amplicon 3144 - 48ux
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 48ux: 5'-TTATGGATCGTGAAGAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7133196
   
wt amplicon (over InsPos) 45vl - 48ux
wt amplicon start Chr1:7133196
wt amplicon end Chr1:7133977
wt amplicon size 782 bp
Locus specific primer 48ux: 5'-TTATGGATCGTGAAGAGG-3'
2nd locus specific primer 45vl: 5'-ACAGCACATTATTCGCAAAGAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11