Genotyping and primer details


Genotyping and primer details for 171F07 - At3g30300
Line ID 171F07
Locus At3g30300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:11923673 of line 171F07 Chr3:11923673
   
Insertion allele amplicon 8474 - NR38
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NR38: 5'-CACAGATGACTCAATCTCCGATTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:11924144
   
wt amplicon (over InsPos) 14fw - NR38
wt amplicon start Chr3:11923173
wt amplicon end Chr3:11924144
wt amplicon size 972 bp
Locus specific primer NR38: 5'-CACAGATGACTCAATCTCCGATTA-3'
2nd locus specific primer 14fw: 5'-AACTAGTTCAACGACCGAGTGTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11