Genotyping and primer details


Genotyping and primer details for 211A01 - At1g26700
Line ID 211A01
Locus At1g26700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:9231539 of line 211A01 Chr1:9231539
   
Insertion allele amplicon 8474 - 78wr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 78wr: 5'-GCTATATGATCCGCTCTATGGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:9230551
   
Insertion allele amplicon 8474 - VL32
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer VL32: 5'-CTTACCCTTTGAGGTAACAATGCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:9231903
   
wt amplicon (over InsPos) VL31 - VL32
wt amplicon start Chr1:9230837
wt amplicon end Chr1:9231903
wt amplicon size 1067 bp
Locus specific primer VL32: 5'-CTTACCCTTTGAGGTAACAATGCT-3'
2nd locus specific primer VL31: 5'-GTTGGTTTCATGCTTTTCAACATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11