Genotyping and primer details


Genotyping and primer details for 221F09 - At2g47800
Line ID 221F09
Locus At2g47800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:19579825 of line 221F09 Chr2:19579825
   
Insertion allele amplicon 8474 - 14aq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 14aq: 5'-ACAGCCTTGTGTTAAAACTCACCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:19579328
   
wt amplicon (over InsPos) au37 - 14aq
wt amplicon start Chr2:19579328
wt amplicon end Chr2:19580025
wt amplicon size 698 bp
Locus specific primer 14aq: 5'-ACAGCCTTGTGTTAAAACTCACCT-3'
2nd locus specific primer au37: 5'-ACATAACCCTGCCTAAGCATAGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11