Genotyping and primer details


Genotyping and primer details for 228H05 - At4g30996
Line ID 228H05
Locus At4g30996 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15102343 of line 228H05 Chr4:15102343
   
Insertion allele amplicon 8474 - CP70
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CP70: 5'-GGATCTTTCAATCAAAGCTGTCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15101853
   
wt amplicon (over InsPos) 04nv - CP70
wt amplicon start Chr4:15101853
wt amplicon end Chr4:15102864
wt amplicon size 1012 bp
Locus specific primer CP70: 5'-GGATCTTTCAATCAAAGCTGTCTC-3'
2nd locus specific primer 04nv: 5'-TCCAAGTGACTTTCTTGATCCTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11