Genotyping and primer details


Genotyping and primer details for 255F04 - At1g05370
Line ID 255F04
Locus At1g05370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1570784 of line 255F04 Chr1:1570784
   
Insertion allele amplicon 8474 - 08ve
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 08ve: 5'-CAGAAGCTACATACGCAGAAACAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1571180
   
wt amplicon (over InsPos) 79wa - 08ve
wt amplicon start Chr1:1569989
wt amplicon end Chr1:1571180
wt amplicon size 1192 bp
Locus specific primer 08ve: 5'-CAGAAGCTACATACGCAGAAACAG-3'
2nd locus specific primer 79wa: 5'-TATGATGACGAGAAATCGTCGTAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11