Genotyping and primer details


Genotyping and primer details for 300A12 - At5g06110
Line ID 300A12
Locus At5g06110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1842223 of line 300A12 Chr5:1842223
   
Insertion allele amplicon 8474 - PF55
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PF55: 5'-ATGACCATTGGCTTCTATATGACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1841639
   
wt amplicon (over InsPos) 25km - PF55
wt amplicon start Chr5:1841639
wt amplicon end Chr5:1842713
wt amplicon size 1075 bp
Locus specific primer PF55: 5'-ATGACCATTGGCTTCTATATGACC-3'
2nd locus specific primer 25km: 5'-GCTACAACATGATCACTATGCGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11