Genotyping and primer details


Genotyping and primer details for 313F08 - At5g16120
Line ID 313F08
Locus At5g16120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5266918 of line 313F08 Chr5:5266918
   
Insertion allele amplicon 8474 - 20vo
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 20vo: 5'-TTGTTTTTCAATGTCCTGAGTTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5267448
   
wt amplicon (over InsPos) 41vu - 20vo
wt amplicon start Chr5:5266166
wt amplicon end Chr5:5267448
wt amplicon size 1283 bp
Locus specific primer 20vo: 5'-TTGTTTTTCAATGTCCTGAGTTGT-3'
2nd locus specific primer 41vu: 5'-ATCTCGATCATATCCTCTTCAAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11