Genotyping and primer details


Genotyping and primer details for 352H03 - At3g22410
Line ID 352H03
Locus At3g22410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:7934339 of line 352H03 Chr3:7934339
   
Insertion allele amplicon 8474 - 15ve
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 15ve: 5'-GATATCTAGCGGTTCGTCGTAGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:7933898
   
wt amplicon (over InsPos) 76wa - 15ve
wt amplicon start Chr3:7933898
wt amplicon end Chr3:7934779
wt amplicon size 882 bp
Locus specific primer 15ve: 5'-GATATCTAGCGGTTCGTCGTAGTC-3'
2nd locus specific primer 76wa: 5'-TATTTGCATTTATTTGATGTCCGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11