Genotyping and primer details


Genotyping and primer details for 356A12 - At3g11450
Line ID 356A12
Locus At3g11450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3605924 of line 356A12 Chr3:3605924
   
Insertion allele amplicon 8474 - 10lc
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 10lc: 5'-GAATCTGCACAACAACAAAAGAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3606364
   
wt amplicon (over InsPos) 02nl - 10lc
wt amplicon start Chr3:3605490
wt amplicon end Chr3:3606364
wt amplicon size 875 bp
Locus specific primer 10lc: 5'-GAATCTGCACAACAACAAAAGAAA-3'
2nd locus specific primer 02nl: 5'-TCCTTAAGCTCTGCAAACTTCTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11