Genotyping and primer details


Genotyping and primer details for 383G06 - At5g05950
Line ID 383G06
Locus At5g05950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1788846 of line 383G06 Chr5:1788846
   
Insertion allele amplicon 8474 - KP70
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KP70: 5'-CAGAAGCCAAACCAAGAGATTTAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1789466
   
wt amplicon (over InsPos) AE05 - KP70
wt amplicon start Chr5:1788398
wt amplicon end Chr5:1789466
wt amplicon size 1069 bp
Locus specific primer KP70: 5'-CAGAAGCCAAACCAAGAGATTTAC-3'
2nd locus specific primer AE05: 5'-TAATGCAGACATGGATTGATTTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11