Genotyping and primer details


Genotyping and primer details for 405G07 - At5g48160
Line ID 405G07
Locus At5g48160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:19528338 of line 405G07 Chr5:19528338
   
Insertion allele amplicon 8474 - 32tg
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 32tg: 5'-TGGAGAAGGTGGACGTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19528613
   
wt amplicon (over InsPos) 01ue - 32tg
wt amplicon start Chr5:19528030
wt amplicon end Chr5:19528613
wt amplicon size 584 bp
Locus specific primer 32tg: 5'-TGGAGAAGGTGGACGTTT-3'
2nd locus specific primer 01ue: 5'-ATCTGAAAGGATTACGCTCGTTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11