Genotyping and primer details


Genotyping and primer details for 461B08 - At1g18180
Line ID 461B08
Locus At1g18180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6257670 of line 461B08 Chr1:6257670
   
Insertion allele amplicon 8474 - 25ny
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 25ny: 5'-CAAAGTTTAAACCAGCTTTCCAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6258234
   
wt amplicon (over InsPos) 70rd - 25ny
wt amplicon start Chr1:6257276
wt amplicon end Chr1:6258234
wt amplicon size 959 bp
Locus specific primer 25ny: 5'-CAAAGTTTAAACCAGCTTTCCAAG-3'
2nd locus specific primer 70rd: 5'-AATCACACAGCAGAAACAACATTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11