Genotyping and primer details


Genotyping and primer details for 541F02 - At1g53450
Line ID 541F02
Locus At1g53450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19953552 of line 541F02 Chr1:19953552
   
Insertion allele amplicon 8474 - 22vh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 22vh: 5'-CTCTCACAGGGGAATTTGAAGTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19952930
   
wt amplicon (over InsPos) 30vq - 22vh
wt amplicon start Chr1:19952930
wt amplicon end Chr1:19954174
wt amplicon size 1245 bp
Locus specific primer 22vh: 5'-CTCTCACAGGGGAATTTGAAGTAG-3'
2nd locus specific primer 30vq: 5'-ATTTGTTTGCGTCTTTCTTCACTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11