Genotyping and primer details


Genotyping and primer details for 556A06 - At5g62070
Line ID 556A06
Locus At5g62070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:24932161 of line 556A06 Chr5:24932161
   
Insertion allele amplicon 8474 - 26ew
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 26ew: 5'-TAAATGGTTTGAACAAAACCACAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24932793
   
wt amplicon (over InsPos) 04fq - 26ew
wt amplicon start Chr5:24931618
wt amplicon end Chr5:24932793
wt amplicon size 1176 bp
Locus specific primer 26ew: 5'-TAAATGGTTTGAACAAAACCACAA-3'
2nd locus specific primer 04fq: 5'-AAAATCAAATGATTCACACCAACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11