Genotyping and primer details


Genotyping and primer details for 556E04 - At1g32130
Line ID 556E04
Locus At1g32130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:11560184 of line 556E04 Chr1:11560184
   
Insertion allele amplicon 8474 - AY62
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AY62: 5'-TGCCTGAGAGAGAATTTTGTTTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:11561048
   
Insertion allele amplicon 8474 - AY63
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AY63: 5'-TTGCTAGGTCTATCCCCAGTAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:11559774
   
wt amplicon (over InsPos) AY63 - AY62
wt amplicon start Chr1:11559774
wt amplicon end Chr1:11561048
wt amplicon size 1275 bp
Locus specific primer AY62: 5'-TGCCTGAGAGAGAATTTTGTTTTT-3'
2nd locus specific primer AY63: 5'-TTGCTAGGTCTATCCCCAGTAGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11